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Unit 4 Schedule: Recombinant DNA

http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html

date in class homework
9/19

restriction digests

Read Ligation Procedure, read agarose gel protocol

9/21 ligation Read gel documentation protocol
9/22 ligation gel - --LONG DAY Tutorial Answer Gallo questions
9/23

analyze gel results, discuss Gallo

 
 

UNIT 3 - FINDING THE GENE

 

10/4 Pour plates +/- additives (TS) +discuss Klatzmann Read Transformation Protocol
10/5 transformations--LONG DAY Tutorial (CW)  
10/6 analyze transformation results  
10/10

start ON culture, Discuss protein purification

Read Cell Lysate Protocol
10/11

Start cell lysate (dr. w)

 

10/12 finish lysate + affinity chromatography if time  
10/13 Protein electrophoresis theory  
10/17

prepare samples for loading gel

 

10/18

Load and run protein gel

Tutorial

 

 

10/19 destain gel Go to Moodle Page and download Practical Exam Topics
10/25 mount gel, wrap up, start Schnittman

read Schnittman

10/26 review techniques for practical, try experimental design problem review theory and techniques for practical exam
10/27 Lab practical